ELISpot Flex: Porcine IgG (ALP)

ELISpot Flex: Porcine IgG (ALP)

3151-2A

Publications: 4
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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT421
Detection mAbMT424, biotin
Enzyme conjugateStreptavidin-ALP
StimulusR848

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Delivery 4-9 business days

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Complementary products
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ELISpot plates, white


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of B cells secreting porcine IgG using the ELISpot assay. B cells secreting antigen-specific IgG, as well as B cells secreting IgG irrespective of antigen specificity (total IgG) can be enumerated. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 (included).

Recommendation

Product details

ProductELISpot Flex: Porcine IgG (ALP)
ApplicationELISpot
AnalyteIgG
ReactivityPig
Specificity

This kit is based on a matched pair of mAbs specific for porcine (pig) IgG.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. R848 should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Porcine PBMC were pre-incubated for 72 h in the absence or presence of R848. The cells were then washed, added into ELISpot wells and analyzed for the secretion of total IgG (50,000 cells/well, overnight incubation).
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 21, 2022

B cell profiling with ELISpot and FluoroSpot
ELISpot and FluoroSpot are considered the gold standard method for finding rare, antigen-specific B cells.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
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IgG

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG, IGHG, IGHG1, IGHG1.02, IGHJ1, IgG1b
Cell typeB cell
Gene ID396781