ELISpot Flex: Mouse IL‑5 (ALP)

Mouse spleen cells (400,000 cells/well) were incubated for 40h in the absence or presence of Concanavalin A (ConA) plus anti-CD28 antibody and the number of cells secreting IL-5 was analyzed by ELISpot.

ELISpot Flex: Mouse IL‑5 (ALP)

3391-2A

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Publications: 15
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Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbTRFK5
Detection mAbTRFK4, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot

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Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
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ELISpot substrate: BCIP/NBT-plus for ALP

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IL-5 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Mouse IL-5 (ALP)
ApplicationELISpot
AnalyteIL-5
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse IL-5.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells (400,000 cells/well) were incubated for 40h in the absence or presence of Concanavalin A (ConA) plus anti-CD28 antibody and the number of cells secreting IL-5 was analyzed by ELISpot.
Mouse spleen cells (400,000 cells/well) were incubated for 40h in the absence or presence of Concanavalin A (ConA) plus anti-CD28 antibody and the number of cells secreting IL-5 was analyzed by ELISpot.
Datasheet/ProtocolSDS

Tutorial
Published: December 28, 2023 | 2 minute read

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, November 10, 2022

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, October 14, 2022

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
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IL-5

Analyte description

Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells.  IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells.

Alternative namesInterleukin-5, IL-5, IL5, BCGF-2, Il-5
Cell typeT cell, Th2, NK cell
Gene ID16191
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ELISpot Plus: Mouse IL-5 (ALP)