ELISpot Flex: Mouse IL‑4 (HRP)

3311-2H

Publications: 178

Documents

Bulk or custom format

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	11B11
Detection mAb	BVD6-24G2, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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ELISpot substrate: TMB for HRP

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IL-4 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Flex: Mouse IL-4 (HRP)
Application	ELISpot
Analyte	IL-4
Reactivity	Mouse
Specificity	This kit is based on a matched pair of mAbs specific for mouse IL-4.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 18 months from date of receipt.

Datasheet/Protocol SDS

Tutorial

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

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IL-4

Analyte description	Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells.
Alternative names	Interleukin 4, IL-4, IL4, BCGF-1, BSF-1, Il-4
Cell type	T cell, Th2, Treg
Gene ID	16189

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ELISpot Plus: Mouse IL-4 (HRP)