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ELISpot Flex: Mouse IL‑2 (HRP)

ELISpot Flex: Mouse IL‑2 (HRP)

3441-2H

Publications: 88
Documents
Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb1A12
Detection mAb5H4, biotin
Enzyme conjugateStreptavidin-HRP enzyme conjugate

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
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ELISpot substrate: TMB for HRP

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IL-2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Mouse IL-2 (HRP)
ApplicationELISpot
AnalyteIL-2
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse IL-2.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.

Datasheet/ProtocolSDS

Tutorial

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.
Tutorial

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.
Tutorial

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.
Tutorial

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
Tutorial

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

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IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, Il-2
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID16183

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ELISpot Plus: Mouse IL-2 (HRP)