ELISpot Flex: Human IgG1 (ALP)

ELISpot Flex: Human IgG1 (ALP)

3851-2A

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Bulk or custom format

Our most adaptable ELISpot format. Combine it with substrate, plate, antigen of choice, and Mabtech ASTOR 2 – a reader designed around your ELISpot workflow.

Components

Capture mAb	MT145
Detection mAb	MTG1218, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot
Stimuli	R848
	Recombinant human IL-2

In stock

Delivery 4-9 business days

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Frequently bought together

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ELISpot plates, white

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IgG1 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

With this kit, detection of antigen-specific IgG1 secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody.

For analysis of memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included)."

Recommendation

Product details

Product	ELISpot Flex: Human IgG1 (ALP)
Application	ELISpot
Analyte	IgG1
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of human IgG1.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. R848 and IL-2 should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

PBMC were pre-incubated in the presence or absence of R848 and recombinant IL-2. The cells were then washed, added into ELISpot wells (100,000 cells/well) and analyzed for the secretion of IgG1 (overnight incubation).

Datasheet/Protocol SDS

Tutorial

2022-11-24

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In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

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B cell profiling with ELISpot and FluoroSpot

ELISpot and FluoroSpot are considered the gold standard method for finding rare, antigen-specific B cells.

Tutorial

2022-11-10

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial

2022-11-07

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial

2022-10-14

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial

2022-09-02

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

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IgG1

Analyte description	Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with the highest serum concentrations of IgG1, followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. IgG1 binds to Fc receptors with high affinity and may also activate the complement system.
Alternative names	Immunoglobulin G1, IgG1
Cell type	B cell
Gene ID	3500

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Anti-human IgG1 mAb (MTG1218), unconjugated