ELISpot Flex: Human IgG1 (ALP)

3851-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT145
Detection mAb	MTG1218, biotin
Enzyme conjugate	Streptavidin-ALP
Stimuli	R848
	Recombinant human IL-2

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Delivery 4-9 business days

Shipping $0

Complementary products

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ELISpot plates, white

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IgG1 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

With this kit, detection of antigen-specific IgG1 secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody.

For analysis of memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included)."

Recommendation

Product details

Product	ELISpot Flex: Human IgG1 (ALP)
Application	ELISpot
Analyte	IgG1
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of human IgG1.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. R848 and IL-2 should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Performance

PBMC were pre-incubated in the presence or absence of R848 and recombinant IL-2. The cells were then washed, added into ELISpot wells (100,000 cells/well) and analyzed for the secretion of IgG1 (overnight incubation).

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 21, 2022

B cell profiling with ELISpot and FluoroSpot

ELISpot and FluoroSpot are considered the gold standard method for finding rare, antigen-specific B cells.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Publications (0)

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Analyte information

IgG1

Analyte description	Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with the highest serum concentrations of IgG1, followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. IgG1 binds to Fc receptors with high affinity and may also activate the complement system.
Alternative names	Immunoglobulin G1, IgG1
Cell type	B cell
Gene ID	3500