ELISpot Flex: Ferret IL-2 (ALP)

ELISpot Flex: Ferret IL-2 (ALP)

3104-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT264/271
Detection mAbMT265, biotin
Enzyme conjugateStreptavidin-ALP

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Delivery 4-9 business days

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ELISpot substrate: BCIP/NBT-plus for ALP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting ferret IL-2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Ferret IL-2 (ALP)
ApplicationELISpot
AnalyteIL-2
ReactivityFerret
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of ferret IL-2.
Cross-reactivityThe mAbs react with pig IL-2 and cross-react with IL-2 from ferret. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
Ferret spleen cells (300,000 cells/well) were incubated for 20 h in the absence or presence of PHA and the number of cells secreting IL-2 was analyzed by ELISpot.
Datasheet/Protocol
SDS

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IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, POIL2, TCGF
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID396868, 101674110