ELISpot Flex: Bovine IL‑8 (ALP)

3114-2A

Publications: 1

Documents

Bulk or custom format

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT8H6/8F19
Detection mAb	26E5, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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ELISpot substrate: BCIP/NBT-plus for ALP

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting bovine IL-8 (CXCL8) using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Flex: Bovine IL-8 (ALP)
Application	ELISpot
Analyte	IL-8 (CXCL8)
Reactivity	Cow, Dog
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of bovine (cow) IL-8 (CXCL8).
Cross-reactivity	The mAbs cross-react with IL-8 from dog. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 18 months from date of receipt.

Datasheet/Protocol SDS

Tutorial

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

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IL-8 (CXCL8)

Analyte description	Interleukin 8 (IL-8) is a proinflammatory cytokine produced by different types of cells, including monocytes, macrophages, and endothelial cells. Inflammatory stimuli, such as LPS, strongly induce IL-8 production by monocytes.
Alternative names	Interleukin-8, IL-8, IL8, CXCL8
Cell type	T cell, Treg, Tfh, Monocyte/MΦ, mDC
Gene ID	280828, 403850

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ELISpot Plus: Bovine IL-8 (ALP)