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ELISpot Flex: Bovine IgG (ALP)

Bovine PBMC were pre-incubated in the absence or presence of R848. The cells were then washed, added into ELISpot wells in serum free medium, and analyzed for the secretion of total IgG (overnight incubation).

ELISpot Flex: Bovine IgG (ALP)

3150-2A

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Bulk or custom format
Our most adaptable ELISpot format. Combine it with substrate, plate, antigen of choice, and Mabtech ASTOR 2 – a reader designed around your ELISpot workflow.

Components

Capture mAbMT134
Detection mAbMT391, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
StimulusR848

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Delivery 4-9 business days

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ELISpot plates, white

Intended use

This ELISpot Flex kit is intended for the enumeration of B cells secreting bovine IgG using the ELISpot assay. B cells secreting antigen-specific IgG, as well as B cells secreting IgG irrespective of antigen specificity (total IgG) can be enumerated. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 (included).

Recommendation

Product details

ProductELISpot Flex: Bovine IgG (ALP)
ApplicationELISpot
AnalyteIgG
ReactivityCow
Specificity

This kit is based on a matched pair of mAbs specific for bovine (cow) IgG.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. R848 should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.

Bovine PBMC were pre-incubated in the absence or presence of R848. The cells were then washed, added into ELISpot wells in serum free medium, and analyzed for the secretion of total IgG (overnight incubation).
Bovine PBMC were pre-incubated in the absence or presence of R848. The cells were then washed, added into ELISpot wells in serum free medium, and analyzed for the secretion of total IgG (overnight incubation).

Datasheet/ProtocolSDS

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IgG

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG
Cell typeB cell
Gene ID281850, 281851, 281852