ELISA Flex: Human IL‑1β (HRP)

Standard curve for human Interleukin-1beta (IL-1β) ELISA Flex kit.

ELISA Flex: Human IL‑1β (HRP)

3416-1H-6

Publications: 47

Documents

Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MT175
Detection mAb	7P10, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate
Standard	Recombinant human IL-1β ELISA standard
Buffer/Solution	Standard reconstitution buffer

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Delivery 4-9 business days

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Frequently bought together

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ELISA diluent

Intended use

This ELISA Flex kit is intended for the quantification of human IL-1β in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Human IL-1β (HRP)
Application	ELISA
Analyte	IL-1β
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-1β (pro-form and active form).
Cross-reactivity	The mAbs do not cross-react with human IL-1α. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Standard range

2.5-250 pg/ml

Representative standard curve for human IL-1β ELISA Flex kit using TMB substrate.

Datasheet/Protocol SDS

Tutorial

2025-04-08

11 tips for your first ELISA

Get the most out of your next ELISA experiment and find out how to get reliable results with our checklist.

Tutorial

2023-09-20

ELISA kit with HRP or ALP – what to choose?

You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial

2022-11-28

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

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IL-1β

Analyte description	Interleukin 1ß (IL-1ß) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.
Alternative names	Interleukin-1ß, IL-1ß, IL-1F2, Interleukin-1beta, IL-1 beta, IL1b, Interleukin-1 beta, IL-1, IL1-BETA, IL1F2, IL1beta
Cell type	Monocyte/MΦ, mDC
Gene ID	3553

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ELISA Pro: Human IL-1β