ELISA PathRF: Human IL‑1β

3416-1HP-DP-1

$615

Publications: 0

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Bulk or custom format

Optimized to block rheumatoid factor and therefore suitable when studying samples from patients with autoimmune disease.

Components

Plate	Pre-coated ELISA strip plate (mAb MT175)
Detection mAb	MTR-4, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate
Standard	Recombinant human IL-1β ELISA standard
Substrate	TMB substrate for ELISA
Buffers/Solutions	RF-block diluent
	Standard reconstitution buffer
	Streptavidin-HRP diluent
	Wash buffer concentrate
	Stop solution
Also provided	Adhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA PathRF kit is intended for the quantification of human IL-1β in serum and plasma containing rheumatoid factor (RF). For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

ELISA PathRF kits
ELISA PathRF kits are designed to eliminate potential interference from RF and heterophilic antibodies. The combination of a recombinant detection mAb and RF-block diluent prevents RF and heterophilic antibodies from cross-linking the assay antibodies, effectively reducing the risk of false-positive signal. RF and heterophilic antibodies can be found at high concentrations in serum and plasma from individuals with autoimmune diseases such as rheumatoid arthritis. The kit has been validated with RF-containing plasma.

Recommendation

Product details

Product	ELISA PathRF: Human IL-1β
Application	ELISA
Analyte	IL-1β
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-1β (pro-form and active form).
Cross-reactivity	The mAbs do not cross-react with human IL-1α. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Standard range	1-316 pg/ml
Sensitivity	0.4 pg/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 2 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of ELISA PathRF: Human IL-1β.

Documents

Datasheet/Protocol SDS

Tutorials

Tutorial, April 8, 2025

11 tips for your first ELISA

Get the most out of your next ELISA experiment and find out how to get reliable results with our checklist.

Tutorial, November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Product highlights

How to mitigate interference caused by rheumatoid factor

Analyzing samples from patients with ELISA? Watch out for heterophilic antibodies causing false positives. Discover how Mabtech’s kits overcome this issue.

Publications (0)

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Analyte information

IL-1β

Analyte description	Interleukin 1ß (IL-1ß) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.
Alternative names	Interleukin-1ß, IL-1ß, IL-1F2, Interleukin-1beta, IL-1 beta, IL1b, Interleukin-1 beta, IL-1, IL1-BETA, IL1F2, IL1beta
Cell type	Monocyte/MΦ, mDC
Gene ID	3553