ELISA substrate: TMB for HRP

ELISA substrate: TMB for HRP


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Ready-to-use TMB substrate for ELISA 

In stock

Delivery 4-9 business days

Shipping $0

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Product specifications

Intended use

This ELISA substrate is intended for the development of ELISA assays using Streptavidin-HRP enzyme conjugates. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

This high sensitivity TMB substrate is suitable for ELISA assays with horseradish peroxidase (HRP). The substrate is supplied as a ready-to-use solution and produces a soluble blue product. Addition of an acid, e.g. sulfuric acid, stops the enzymatic reaction, which changes the color from blue to yellow. The absorbance of the stopped reaction is measured at 450 nm.


The substrate should preferably be used cold. Add the substrate after incubation with horseradish peroxidase and subsequent washing. Incubate for typically 5-15 minutes and then stop the enzymatic reaction by adding an acid, e.g. 0.18 M H2SO4. This will change the color from blue to yellow. Read the absorbance at 450 nm.

Product details

ProductELISA substrate: TMB for HRP

Ready-to-use TMB substrate for ELISA 

Shipping and Storage


Shipped at ambient temperature.


Store at 4-8 °C upon receipt.

Shelf lifeAt least 12 months from date of receipt.
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