Anti-human EBI3 mAb (MT23A14), unconjugated

3459-3-250

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Anti-human EBI3 mAb (MT140), biotin

Intended use

This monoclonal antibody enables specific detection of the EBI3 subunit of human IL-27 and IL-35 in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

MT23A14 is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb MT140 (product code 3458-6).

Product details

Product	Anti-human EBI3 mAb (MT23A14), unconjugated
Application	ELISpot, FluoroSpot, ELISA
Analyte	EBI3
Antibody	MT23A14
Conjugate	Unconjugated
Clonality	Monoclonal
Immunogen	Recombinant human IL-27
Host	Mouse
Isotype	IgG1
Reactivity	Human
Specificity	Native and recombinant human EBI3. MT23A14 binds to the EBI3 subunit of IL-27 and IL-35.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT23A14, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Datasheet/Protocol SDS

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EBI3

Analyte description	EBI3 is a protein subunit that forms heterodimers with other proteins in the IL-12 family. IL-27 is a heterodimer composed of the subunits EBI3 and p28. IL-35 is a heterodimer composed of the subunits EBI3 and p35. IL-39 is a heterodimer consisting of the subunits EBI3 and p19. Native IL-39 has not yet been detected in humans. The EBI3 has ca. 30% sequence similarity to IL-12 p40.
Alternative names	Epstein–Barr virus-induced gene3, EBI3, Interleukin-27 subunit beta, IL-27B, IL27B, IL35B
Cell type	Monocyte/MΦ, mDC
Gene ID	10148

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ELISA Flex: Human IL-27 (HRP)