Anti-human EBI3 mAb (MT140), biotin

3458-6-250

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Monoclonal antibody MT140, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-human IL-27 mAbs (MT27/361), unconjugated

Product specifications

Intended use

This monoclonal antibody enables specific detection of human IL-27 and the EBI3 subunit of IL-27 and IL-35 in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

MT140 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA for analysis of: IL-27 in combination with capture mAbs MT27/361 (product code 3458-3), and for EBI3 in combination with capture mAb MT23A14 (product code 3459-3).

Product details

Product	Anti-human EBI3 mAb (MT140), biotin
Application	ELISpot, FluoroSpot, ELISA
Analyte	IL-27, EBI3
Antibody	MT140
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Recombinant human IL-27
Host	Mouse
Isotype	IgG1
Reactivity	Human
Specificity	Native and recombinant human EBI3. MT140 binds to the EBI3 subunit of IL-27 and IL-35.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT140, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

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Analyte information

IL-27

Analyte description	The cytokine interleukin 27 (IL-27) is a member of the IL-12 family. IL-27 is a heterodimer composed of one EBI3 subunit and one p28 subunit. Monocytes, endothelial cells, dendritic cells, and T cells produce it. IL-27 has been described to have both pro-inflammatory and anti-inflammatory properties.
Alternative names	Interleukin 27, IL-27, IL27, IL-27AA, IL27p28, IL30, p28
Cell type	Monocyte/MΦ, mDC, NK cell
Gene ID	246778

EBI3

Analyte description	EBI3 is a protein subunit that forms heterodimers with other proteins in the IL-12 family. IL-27 is a heterodimer composed of the subunits EBI3 and p28. IL-35 is a heterodimer composed of the subunits EBI3 and p35. IL-39 is a heterodimer consisting of the subunits EBI3 and p19. Native IL-39 has not yet been detected in humans. The EBI3 has ca. 30% sequence similarity to IL-12 p40.
Alternative names	Epstein–Barr virus-induced gene3, EBI3, Interleukin-27 subunit beta, IL-27B, IL27B, IL35B
Cell type	Monocyte/MΦ, mDC