Anti-human apoA1 mAb (HDL 110), unconjugated

Anti-human apoA1 mAb (HDL 110), unconjugated

3710-3-250

Publications: 58
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Content

Monoclonal antibody HDL 110, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

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Anti-human apoA1 mAb (HDL 44), biotin
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  • Code: 3710-6-250
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Product specifications

Intended use

This monoclonal antibody enables specific detection of apoA1 in immunoassays such as ELISA and Western blot.

Serum/Plasma samples

Recommendation

HDL 110 is recommended as capture mAb in ELISA in combination with detection mAb HDL 44 (product code 3710-6). The antibody is also suitable for immunoprecipitation and Western blot.

Product details

ProductAnti-human apoA1 mAb (HDL 110), unconjugated
ApplicationELISA, Western blot
AnalyteapoA1
AntibodyHDL 110
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenNative human HDL
HostMouse
IsotypeIgG1
ReactivityHuman, Non-human primates
Specificity

Human apolipoprotein A1 (apoA1), purified or in the form of HDL. The presence of low amounts of detergent is necessary for binding. Tween 20, Triton X100 or NP40 can be used at a concentration of 0.01-0.5%.

Cross-reactivityCross-reacts with apoA1 from rhesus and cynomolgus macaques. For more information please check the Cross-reactivity guide section below.
Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration1 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody HDL 110, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Detection of human apoA1 with mAb HDL110 in Western blot. Human plasma (0.1 µl and 0.01 µl) in SDS sample buffer were denatured at 70˚C for 10 minutes and run on SDS-page / Western blot. ApoA1 was detected using 1 µg/ml HDL110 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.
Datasheet/ProtocolSDS

apoA1

Analyte descriptionApolipoprotein A1 (apoA1, also known as apolipoprotein-A1, apo A1 and apoA-I) is the main protein component of high density lipoprotein particles (HDL). ApoA1 (28 kD) is mainly produced in the liver and exported into the circulation where it acquires lipids and becomes HDL. High level of apoA1 is regarded as protective against atherosclerosis and cardiovascular disease. Normal concentration of apoA1 in human plasma is approximately 0.8-2.2 g/L.
Alternative names, HPALP2, apo(a)
Gene ID335
Loading publications...

Tutorial, Published November 14, 2022

Apolipoprotein dilution
Recommendations for Mabtech's apolipoprotein ELISAs

Cross-reactivity guide, Published November 21, 2022

Non-human primate cross-reactivity
Mabtech offers a broad range of ELISpot kits in different formats for the detection of cytokines and other immunological effector molecules in samples from non-human primates (NHP).

Highlighted research, Published June 27, 2019

Alzheimer’s research with apolipoprotein ELISA
Mabtech ELISA Pro: human apoA1 and apoE used in Alzheimer's research.