Anti-human apoA1 mAb (HDL 44), biotin

Anti-human apoA1 mAb (HDL 44), biotin


Publications: 58
Validated for our assays.


Monoclonal antibody HDL 44, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

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Anti-human apoA1 mAb (HDL 110), unconjugated
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  • Application:
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  • Code: 3710-3-250
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Product specifications

Intended use

This monoclonal antibody enables specific detection of apoA1 in immunoassays such as ELISA and Western blot.

Serum/Plasma samples


HDL 44 is recommended as detection mAb in ELISA in combination with capture mAb HDL 110 (product code 3710-3). The antibody is also suitable for immunoprecipitation and Western blot.

Product details

ProductAnti-human apoA1 mAb (HDL 44), biotin
ApplicationELISA, Western blot
AntibodyHDL 44
ImmunogenNative human HDL
ReactivityHuman, Non-human primates

Human apolipoprotein A1 (apoA1), purified or in the form of HDL. The presence of low amounts of detergent is necessary for binding. Tween 20, Triton X100 or NP40 can be used at a concentration of 0.01-0.5%.

Cross-reactivityCross-reacts with apoA1 from rhesus and cynomolgus macaques. For more information please check the Cross-reactivity guide section below.

Purified from in vitro cultures by protein G affinity chromatography.


Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration1 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).


Monoclonal antibody HDL 44, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage


Shipped at ambient temperature.


Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Detection of human apoA1 with mAb HDL44 in Western blot. Human plasma (0.1 µl and 0.01 µl) in SDS sample buffer were denatured at 70˚C for 10 minutes and run on SDS-page / Western blot. ApoA1 was detected using 1 µg/ml HDL44 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.


Analyte descriptionApolipoprotein A1 (apoA1, also known as apolipoprotein-A1, apo A1 and apoA-I) is the main protein component of high density lipoprotein particles (HDL). ApoA1 (28 kD) is mainly produced in the liver and exported into the circulation where it acquires lipids and becomes HDL. High level of apoA1 is regarded as protective against atherosclerosis and cardiovascular disease. Normal concentration of apoA1 in human plasma is approximately 0.8-2.2 g/L.
Alternative names, HPALP2, apo(a)
Gene ID335
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