ELISpot Path: EBV Human IFN-γ

ELISpot Path: EBV Human IFN-γ

3420-2AST-P5-1

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A straightforward setup for functional analysis of a specific scientific question.

Components

Detection mAb7-B6-1, ALP
SubstrateBCIP/NBT-plus
PlatePre-coated ELISpot plate, strip (mAb 1-D1K)
Positive controlAnti-CD3 mAb (CD3-2)
Peptide poolEBV (CD4 and CD8), human

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human IFN-γ in response to EBV peptides using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
The EBV defined peptide pool contains 100 peptides derived from 22 of the latent and lytic EBV proteins. Of the peptides, 66 are MHC class I restricted, and 34 are MHC class II restricted.

Recommendation

Product details

ProductELISpot Path: EBV Human IFN-γ
ApplicationELISpot
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Human PBMC (250,000 cells/well) were incubated overnight in the absence or presence of the EBV defined peptide pool (Mabtech) and the number of cells secreting IFN-γ was analyzed by ELISpot.
Datasheet/Protocol
SDS

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458