Catalog / Sheep IL-17A ELISpot kits
Sheep IL-17A ELISpot kits
Pick the right ELISpot kit for Sheep IL-17A. Our kits differ in what we put in the box and how you develop the signal – this page explains what those differences mean.
IL-17A ELISpot kits are also available for bovine, goat, human, monkey, mouse, and porcine.
What differs between these kits
Whether a pre-coated plate is included
Some of our kits arrive with the plate already coated with the capture antibody, so you can start with your samples. Others supply the antibodies and standard only. Coating your own plate is more work, and it gives you control over the coating conditions and the plate.
Which detection system
Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.
Supplied without a plate, streptavidin conjugate, enough for 4 plates
With a pre-coated white plate, streptavidin conjugate, 2 plates
With a pre-coated white plate, streptavidin conjugate, 10 plates
About IL-17A
These Sheep IL-17A ELISpot kits target IL17A (Bos taurus and Ovis aries).
Interleukin 17A (IL-17A) is a potent proinflammatory cytokine produced by activated Th17 (T helper 17) cells and certain cells belonging to the innate immune system. In mice, IL-17 has also been shown to be produced by activated CD8 T cells and γδ T cells. Th17 cells play an important role in autoimmune diseases and protection against bacteria and fungi. IL-17A acts on a broad range of cell types to induce the expression of cytokines, chemokines, and metalloproteinases. As a result, secretion of IL-17A promotes inflammatory responses, which leads to the recruitment of neutrophils, enhancement of antibody production, and activation of T cells. Increased expression of IL-17A is seen in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. It is also associated with asthma, psoriasis, cancer, and transplant rejection.
Find the right kit
Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.
Pre-coated plate included
Or coat your own
Kit detection system
Which enzyme conjugate we supply
Next, choose whether you want a pre-coated plate.
Frequently bought together
What customers usually order alongside these kits.
Related reading
Guides and tutorials covering Sheep IL-17A and the ELISpot method.
