Catalog / Rabbit IgG ELISpot kits

Rabbit IgG ELISpot kits

Pick the right ELISpot kit for Rabbit IgG. Our kits differ in how you develop the signal – this page explains what those differences mean.

IgG ELISpot kits are also available for bovine, human, mouse, and porcine.

What differs between these kits

Which detection system

Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.

Supplied without a plate, streptavidin conjugate, enough for 4 plates

Supplied without a plate, streptavidin conjugate, enough for 4 plates

About IgG

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Also known asImmunoglobulin G, IgG
Produced byB cell
ReactivityRabbit

Find the right kit

Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.

Kit detection system

Which enzyme conjugate we supply


Choose a detection system to continue.

Frequently bought together

What customers usually order alongside these kits.

ELISpot substrate: BCIP/NBT-plus for ALP

Substrates

ELISpot substrate: BCIP/NBT-plus for ALP

Related reading

Guides and tutorials covering Rabbit IgG and the ELISpot method.

Tutorials

Tutorial

2022-11-24

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.