Catalog / Mouse IgEa ELISpot kits

Mouse IgEa ELISpot kits

Pick the right ELISpot kit for Mouse IgEa. Our kits differ in how you develop the signal – this page explains what those differences mean.

What differs between these kits

Which detection system

Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.

Supplied without a plate, streptavidin conjugate, enough for 4 plates

Supplied without a plate, streptavidin conjugate, enough for 4 plates

About IgEa

These Mouse IgEa ELISpot kits target Ighe (Mus musculus, Gene ID 380792).

Immunoglobulin E (IgE) E is secreted by plasma cells as a monomer during the adaptive immune response. IgE antibodies are believed to play an important role in the immune defense against parasitic infections and in hypersensitivity type I reactions upon re-exposure to a specific allergen by binding to and cross-linking Fc receptors (FcεRI) expressed on mast cells and basophils. Allergen-specific IgE is the basis for many in vitro assays for allergy diagnosis. In mice, two allotypes exist: IgEa and IgEb. IgEa differs by 12 single-base changes from IgEb and is present in different mouse strains.

Also known asImmunoglobulin Ea, IgEa
Produced byB cell
ReactivityMouse
Gene ID380792

Find the right kit

Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.

Kit detection system

Which enzyme conjugate we supply


Choose a detection system to continue.

Frequently bought together

What customers usually order alongside these kits.

ELISpot substrate: BCIP/NBT-plus for ALP

Substrates

ELISpot substrate: BCIP/NBT-plus for ALP

Related reading

Guides and tutorials covering Mouse IgEa and the ELISpot method.

Tutorials

Tutorial

2022-11-24

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.