Catalog / Human IgM ELISpot kits
Human IgM ELISpot kits
Pick the right ELISpot kit for Human IgM. Our kits differ in how you develop the signal – this page explains what those differences mean.
IgM ELISpot kits are also available for mouse.
What differs between these kits
Which detection system
Available with Streptavidin-ALP or Streptavidin-HRP. You add the streptavidin conjugate as a second incubation after the biotinylated antibody, which amplifies the signal. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) perform equivalently, so that choice usually comes down to the substrate and reader you already work with.
Supplied without a plate, streptavidin conjugate, enough for 4 plates
About IgM
These Human IgM ELISpot kits target IGHM (Homo sapiens, Gene ID 3507).
Immunoglobulin M (IgM) is the first antibody isotype (also called class) secreted by the adaptive immune system in response to a foreign antigen. Plasma B cells secrete IgM mainly as pentamers, i.e., five IgM molecules joined together by the J chain. Because IgM is formed early in the immune response and is later replaced by IgG, specific antibodies of the IgM class are characteristic of a recent, ongoing, or chronic infection. Therefore, IgM represents the isotype with the lowest affinity for antigens. Due to its structure, with 10 antigen-binding sites per IgM pentamer, it can nevertheless bind surface-bound antigens with very high avidity. Immunoglobulin M constitutes approximately 10% of the total immunoglobulin concentration in serum. Resting mature yet naive, B lymphocytes express monomeric IgM as a transmembrane antigen receptor that functions as part of the B cell receptor (BCR).
| Also known as | Immunoglobulin M, IgM |
| Produced by | B cell |
| Reactivity | Human and Non-human primates |
| Gene ID | 3507 |
Find the right kit
Choose what your experiment needs. When your choices match a single kit, we'll link you to its product page for prices and ordering.
Kit detection system
Which enzyme conjugate we supply
Choose a detection system to continue.
Frequently bought together
What customers usually order alongside these kits.
Related reading
Guides and tutorials covering Human IgM and the ELISpot method.
