ELISpot Plus: Rabbit IFN-γ (HRP)

ELISpot Plus: Rabbit IFN-γ (HRP)

3110-4HPW-2

Publications: 8
Documents
Questions?
The easiest way to achieve reproducibility in ELISpot.

Components

Detection mAbMT318, biotin
Enzyme conjugateStreptavidin-HRP
SubstrateELISpot substrate: TMB for HRP
PlatePre-coated ELISpot plate, white MSIP (mAb MT327)

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting rabbit IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Rabbit IFN-γ (HRP)
ApplicationELISpot
AnalyteIFN-γ
ReactivityRabbit
SpecificityThis kit is based on a matched pair of mAbs specific for rabbit IFN-γ.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
Rabbit PBMC (100,000 cells/well) were incubated for 20 h in the presence or absence of Phytohaemagglutinin (PHA) and the number of cells secreting IFN-γ was analyzed by ELISpot.
Datasheet/Protocol
SDS

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Tutorial, Published November 7, 2022

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Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell typeT cell, Tc, Th1, NK cell
Gene ID100008602