ELISpot Plus: Mouse IL-5 (ALP)

3391-4APW-2

Publications: 12

Documents

Questions?

The easiest way to achieve reproducibility in ELISpot.

Components

Detection mAb	TRFK4, biotin
Enzyme conjugate	Streptavidin-ALP
Substrate	BCIP/NBT-plus
Plate	Pre-coated ELISpot plate, white MSIP (mAb TRFK5)

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse IL-5 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Plus: Mouse IL-5 (ALP)
Application	ELISpot
Analyte	IL-5
Reactivity	Mouse
Specificity	This kit is based on a matched pair of mAbs specific for mouse IL-5.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 18 months from date of receipt.

Performance

Mouse spleen cells (400,000 cells/well) were incubated for 40h in the absence or presence of Concanavalin A (ConA) plus anti-CD28 antibody and the number of cells secreting IL-5 was analyzed by ELISpot.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published September 7, 2023

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

Publications (12)

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Analyte information

IL-5

Analyte description	Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells. IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells.
Alternative names	Interleukin-5, IL-5, IL5, BCGF-2, Il-5
Cell type	T cell, Th2, NK cell
Gene ID	16191