ELISpot Plus: Mouse IL-1α (ALP)

ELISpot Plus: Mouse IL-1α (ALP)

3417-4APW-2

Publications: 0
Documents
Questions?
The easiest way to achieve reproducibility in ELISpot.

Components

Detection mAbMT951, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus
PlatePre-coated ELISpot plate, white MSIP (mAb MT946)

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse IL-1α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Mouse IL-1α (ALP)
ApplicationELISpot
AnalyteIL-1α
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for mouse IL-1α.

Cross-reactivity

The mAbs do not cross-react with mouse IL-1β.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells were incubated for 20 hours in an ELISpot plate. Cells were unstimulated (200,000 cells/well) or stimulated with LPS (50,000 cells/well). The number of cells secreting IL-1α was analyzed by ELISpot.
Datasheet/Protocol
SDS

Tutorial, Published September 7, 2023

Step-by-step guide to ELISpot
An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes
This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
Loading publications...

IL-1α

Analyte description

Interleukin 1α (IL-1α) is a pro-inflammatory cytokine primarily produced by monocytes, macrophages, and neutrophils. IL-1α induces acute phase responses such as fever, T cell, and macrophage activation. 

Alternative namesInterleukin 1α, IL-1α, hematopoietin 1, Interleukin-1alpha, IL-1 alpha, IL1α, Interleukin-1 alpha, Il-1a
Cell typeMonocyte/MΦ, mDC
Gene ID16175