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FluoroSpot Plus: Mouse IL‑2 (550)

Mouse IL-2 FluoroSpot. Splenocytes were incubated overnight in the absence or presence of ConA. Analysis was made with Mabtech IRIS.

FluoroSpot Plus: Mouse IL‑2 (550)

FSP-42B-2

Publications: 14
Documents
Bulk or custom format
Ensure reproducibility when diving into a multiplex analysis.

Components

PlatePre-coated FluoroSpot plate (mAb 1A12)
Detection mAbAnti-IL-2 mAb (5H4), biotin
Fluorophore conjugateSA-550
Buffer/SolutionFluoroSpot enhancer
Co-stimulusAnti-CD28 mAb

In stock

Delivery 4-9 business days

Shipping $0

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting mouse IL-2 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductFluoroSpot Plus: Mouse IL-2 (550)
ApplicationFluoroSpot
AnalyteIL-2
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse IL-2.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, fluorophore conjugate, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 12 months from date of receipt.

Mouse IL-2 FluoroSpot. Splenocytes were incubated overnight in the absence or presence of ConA. Analysis was made with Mabtech IRIS.
Splenocytes (50,000 cells/well) were incubated overnight in the absence or presence of ConA and the number of cells secreting IL-2 was analyzed by FluoroSpot. Spot analysis was made with Mabtech IRIS.

Datasheet/ProtocolSDS

Tutorial

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.
Tutorial

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
Tutorial

Step-by-step guide to FluoroSpot

An in-depth guide to FluoroSpot, including best practice and tricks we've learnt over the years.

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IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, Il-2
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID16183

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