FluoroSpot Plus: Mouse IFN‑γ (490)

Mouse IFN-γ FluoroSpot (490). Spleen cells stimulated with ConA and anti-CD28. FluoroSpot was analyzed with Mabtech IRIS.

FluoroSpot Plus: Mouse IFN‑γ (490)

FSP-31A-2

Publications: 27

Documents

Bulk or custom format

Ensure reproducibility when diving into a multiplex analysis.

Components

Plate	Pre-coated FluoroSpot plate (mAb AN18)
Detection mAb	Anti-IFN-γ mAb (MTR7), BAM
Fluorophore conjugate	Anti-BAM mAb, 490
Buffer/Solution	FluoroSpot enhancer

In stock

Delivery 4-9 business days

Shipping $0

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting mouse IFN-γ using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	FluoroSpot Plus: Mouse IFN-γ (490)
Application	FluoroSpot
Analyte	IFN-γ
Reactivity	Mouse
Specificity	This kit is based on a matched pair of mAbs specific for mouse IFN-γ.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, fluorophore conjugate, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Mouse spleen cells (100,000 cells/well) were incubated for 24 hours in the FluoroSpot plate in the absence or presence of ConA and anti-CD28 antibody. IFN-γ spots were analyzed with Mabtech IRIS.

Datasheet/Protocol SDS

Tutorial

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

Tutorial

Step-by-step guide to FluoroSpot

An in-depth guide to FluoroSpot, including best practice and tricks we've learnt over the years.

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IFN-γ

Analyte description	Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names	Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, If2f, Ifg
Cell type	T cell, Tc, Th1, NK cell
Gene ID	15978

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FluoroSpot Plus: Mouse IFN-γ/IL-2