FluoroSpot Plus: Human IL-6/IL-1β/GM-CSF/TNF-α

FSP-13121509-2

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Ensure reproducibility when diving into a multiplex analysis.

Components

Detection mAbs	Anti-IL-6 mAb (39C3), DIG
	Anti-IL-1β mAb (7P10), BAM
	Anti-GM-CSF mAb (23B6), biotin
	Anti-TNF-α mAb (MT20D9), WASP
Fluorophore conjugates	Anti-DIG mAb, 380
	Anti-BAM mAb, 490
	SA-550
	Anti-WASP mAb, 640
Buffer/Solution	FluoroSpot enhancer
Plate	Pre-coated FluoroSpot plate (mAbs 13A5, MT175, 21C11, and MT25C5)

In stock

Delivery 4-9 business days

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Product specifications

Intended use

This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IL-6, IL-1β, GM-CSF, and TNF-α using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	FluoroSpot Plus: Human IL-6/IL-1β/GM-CSF/TNF-α
Application	FluoroSpot
Analyte	IL-6, IL-1β, GM-CSF, TNF-α
Reactivity	Human
Specificity	This kit is based on matched pairs of mAbs specific for human IL-6, IL-1β, GM-CSF, and TNF-α.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

PBMC (25,000 cells/well) were incubated overnight in the absence or presence of PHA and the number of cells secreting IL-6, IL-1β, GM-CSF, and TNF-α was analyzed by FluoroSpot. Single cytokine secreting cells as well as cells secreting two, three or four analytes were determined. Spot center was used to identify spots from cells secreting multiple analytes, visualized in an image overlay of LED380 (IL-6), LED490 (IL-1β) and LED550, (GM-CSF) and LED640, Cy5 (TNF-α). Spot analysis was made with Mabtech.

Documents

Datasheet/Protocol
SDS

Tutorials

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Analyte information

IL-6

Analyte description	Interleukin 6 (IL-6) is a pleiotropic cytokine produced by many different cell types and plays a role in a wide range of functions, such as immune responses, acute-phase reactions, and hematopoiesis. Among other things, it augments antibody production from activated B cells in vitro.
Alternative names	Interleukin 6, IL-6, IL6, IFB-B502, BSF-2, BCDF, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2
Cell type	B cell, Monocyte/MΦ, mDC
Gene ID	3569

IL-1β

Analyte description	Interleukin 1ß (IL-1ß) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.
Alternative names	Interleukin-1ß, IL-1ß, IL-1F2, Interleukin-1beta, IL-1 beta, IL1b, Interleukin-1 beta, IL-1, IL1-BETA, IL1F2, IL1beta
Cell type	Monocyte/MΦ, mDC
Gene ID	3553

GM-CSF

Analyte description	Granulocyte macrophage-colony stimulating factor (GM-CSF) can be secreted by T cells, macrophages, endothelial cells, and fibroblasts. GM-CSF stimulates the survival and functional activities of myeloid, that is, monocytes, macrophages, DCs, neutrophils, and eosinophils. It also stimulates the differentiation and proliferation of hematological progenitors.
Alternative names	Granulocyte macrophage-colony stimulating factor, GM-CSF, CSF2, CSF, GMCSF
Cell type	T cell
Gene ID	1437

TNF-α

Analyte description	Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.
Alternative names	Tumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell type	T cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID	7124