ELISpot Plus: Porcine IL‑5 (ALP)

Porcine PBMC (500,000 cells/well) were incubated for 96 hours in the presence or absence of PMA/ionomycin and the number of cells secreting IL-5 was analyzed by ELISpot.

ELISpot Plus: Porcine IL‑5 (ALP)

3194-4APW-2

$565

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Bulk or custom format
Pre-coated plates for a more convenient assay.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MTAL25)
Detection mAbMTAL59, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
SubstrateBCIP/NBT-plus substrate for ELISpot

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting porcine IL-5 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Porcine IL-5 (ALP)
ApplicationELISpot
AnalyteIL-5
ReactivityPig
Specificity

This kit is based on a matched pair of mAbs specific for porcine (pig) IL-5.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Porcine PBMC (500,000 cells/well) were incubated for 96 hours in the presence or absence of PMA/ionomycin and the number of cells secreting IL-5 was analyzed by ELISpot.

Porcine PBMC (500,000 cells/well) were incubated for 96 hours in the presence or absence of PMA/ionomycin and the number of cells secreting IL-5 was analyzed by ELISpot.

Datasheet/ProtocolSDS

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IL-5

Analyte description

Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells.  IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells.

Alternative namesInterleukin-5, IL-5, IL5, BCGF-2
Cell typeT cell, Th2, NK cell
Gene ID397409