ELISpot Plus: Mouse IL‑6 (ALP)

Mouse spleen cells (50,000 cells/well) were incubated overnight with LPS or medium only and the number of cells secreting Interleukin-6 (IL-6) was analyzed by ELISpot.

ELISpot Plus: Mouse IL‑6 (ALP)

3361-4APW-2

$565

Publications: 9
Documents
Bulk or custom format
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT26B2)
Detection mAb32C11, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
SubstrateBCIP/NBT-plus substrate for ELISpot

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting mouse IL-6 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Mouse IL-6 (ALP)
ApplicationELISpot
AnalyteIL-6
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse IL-6.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells (50,000 cells/well) were incubated overnight with LPS or medium only and the number of cells secreting Interleukin-6 (IL-6) was analyzed by ELISpot.
Mouse spleen cells (50,000 cells/well) were incubated overnight with LPS or medium only and the number of cells secreting Interleukin-6 (IL-6) was analyzed by ELISpot.
Datasheet/ProtocolSDS

Tutorial
Published: December 28, 2023 | 2 minute read

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, November 10, 2022

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, October 14, 2022

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
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IL-6

Analyte description

Interleukin 6 (IL-6) is a pleiotropic cytokine produced by many different cell types and plays a role in a wide range of functions, such as immune responses, acute-phase reactions, and hematopoiesis. Among other things, it augments antibody production from activated B cells in vitro.

Alternative namesInterleukin 6, IL-6, IL6, IFB-B502, BSF-2, BCDF, Il-6
Cell typeB cell, Monocyte/MΦ, mDC
Gene ID16193