ELISpot Plus: Equine IL‑10 (ALP)

PBMC from horse (50,000 cells/well) were incubated overnight with LPS or medium only and the number of cells secreting IL-10 was analyzed by ELISpot.
PBMC from horse (50,000 cells/well) were incubated overnight with PHA or medium only and the number of cells secreting IL-10 was analyzed by ELISpot.

ELISpot Plus: Equine IL‑10 (ALP)

3126-4APW-2

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Bulk or custom format
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT3515)
Detection mAbMT32AD, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
SubstrateBCIP/NBT-plus substrate for ELISpot

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting equine IL-10 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Equine IL-10 (ALP)
ApplicationELISpot
AnalyteIL-10
ReactivityHorse
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of equine (horse) IL-10.
Cross-reactivityThe mAbs react with monkey IL-10 and cross-react with IL-10 from human and horse. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
PBMC from horse (50,000 cells/well) were incubated overnight with LPS or medium only and the number of cells secreting IL-10 was analyzed by ELISpot.
PBMC from horse (50,000 cells/well) were incubated overnight with LPS or medium only and the number of cells secreting IL-10 was analyzed by ELISpot.
PBMC from horse (50,000 cells/well) were incubated overnight with LPS or medium only and the number of cells secreting IL-10 was analyzed by ELISpot.
PBMC from horse (50,000 cells/well) were incubated overnight with PHA or medium only and the number of cells secreting IL-10 was analyzed by ELISpot.
Datasheet/ProtocolSDS

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IL-10

Analyte description

Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.

Alternative namesInterleukin-10, IL-10, IL10
Cell typeT cell, Treg, Monocyte/MΦ, mDC