ELISpot Flex: Mouse IgG3 (HRP)

ELISpot Flex: Mouse IgG3 (HRP)

3826-2H

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Bulk or custom format

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT24/JC5-1
Detection mAb	MG3, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate
Stimulus	R848

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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Anti-mouse IgG3 mAb (MG3), unconjugated

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IgG3 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

With this kit, detection of antigen-specific IgG3 secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody.

For memory B cells, we recommend pre-stimulation with R848 and recombinant mouse IL-2 (both included).

Recommendation

Product details

Product	ELISpot Flex: Mouse IgG3 (HRP)
Application	ELISpot
Analyte	IgG3
Reactivity	Mouse
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of mouse IgG3.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. R848 and IL-2 should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Mouse IgG3 ELISpot using in vivo activated spleen cells from Balb/c mice (100.000 cells/well).

Datasheet/Protocol SDS

Tutorial

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial

B cell profiling with ELISpot and FluoroSpot

ELISpot and FluoroSpot are considered the gold standard method for finding rare, antigen-specific B cells.

Tutorial

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial

Isolation, freezing, and thawing of splenocytes

This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.

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IgG3

Analyte description	Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. IgG3 binds to Fc receptors with high affinity and is a good complement activator.
Alternative names	Immunoglobulin G3, IgG3
Cell type	B cell
Gene ID	380795