ELISpot Flex: Mouse IgG2c (ALP)

ELISpot Flex: Mouse IgG2c (ALP)

3824-2A

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Bulk or custom format

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT24/JC5-1
Detection mAb	MTG2c, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot
Stimuli	R848
	Recombinant mouse IL-2

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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Anti-mouse IgG2c mAb (MTG2c), unconjugated

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IgG2c using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

With this kit, detection of antigen-specific IgG2c secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody.

The IgG2c allele is expressed in certain inbred mouse strains e.g., C57BL/6, C57BL/10, SJL and NOD.

For memory B cells, we recommend pre-stimulation with R848 and recombinant mouse IL-2 (both included).

Recommendation

Product details

Product	ELISpot Flex: Mouse IgG2c (ALP)
Application	ELISpot
Analyte	IgG2c
Reactivity	Mouse
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of mouse IgG2c.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. R848 and IL-2 should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Splenocytes from C57/BL6 mice were pre-incubated for 72 h in the absence or presence of R848 and recombinant IL-2. The cells were then washed, added into ELISpot wells and analyzed for the secretion of IgG2c (50,000 cells/well, overnight incubation).

Datasheet/Protocol SDS

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Isolation, freezing, and thawing of splenocytes

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IgG2c

Analyte description	IgG (Immunoglobulin G) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG2c allele is expressed (instead of IgG2a) in certain inbred mouse strains, e.g., C57BL/6, C57BL/10, SJL, and NOD.
Alternative names	Immunoglobulin G2c, IgG2c
Cell type	B cell