ELISpot Flex: Mouse IgG2a (HRP)

ELISpot Flex: Mouse IgG2a (HRP)

3822-2H

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT24/JC5-1
Detection mAbMG2a, biotin
Enzyme conjugateStreptavidin-HRP
StimuliR848
Recombinant mouse IL-2

In stock

Delivery 4-9 business days

Shipping $0

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Anti-mouse IgG2a mAb (MG2a), unconjugated


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IgG2a using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

With this kit, detection of antigen-specific IgG2a secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody.

The IgG2a allele is expressed in e.g., Balb/c mice.

For memory B cells, we recommend pre-stimulation with R848 and recombinant mouse IL-2 (both included).

Recommendation

Product details

ProductELISpot Flex: Mouse IgG2a (HRP)
ApplicationELISpot
AnalyteIgG2a
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs enabling specific detection of mouse IgG2a.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. R848 and IL-2 should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Mouse IgG2a ELISpot using in vivo activated spleen cells from Balb/c mice (100.000 cells/well).
Datasheet/Protocol
SDS

Selecting the appropriate mAbs is key to detecting both total and antigen-specific mouse IgG2a. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect mouse IgG2a antibody-secreting cells.

 

Application guide: ELISpot

 

Ag-specific IgG2a

Coating:
Antigen
Detection mAb:
MG2a, biotin

mouse igg2a 1

 

Coating:
MT24/JC5-1, unconjugated
Detection mAb:
Antigen, biotin

mouse igg2a 2

Total IgG2a

Coating:
MT24/JC5-1, unconjugated
Detection mAb:
MG2a, biotin

mouse igg2a 3

 

 

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IgG2a

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In mice, the IgG subclasses are defined IgG1, IgG2a/c, IgG2b, and IgG3.  The IgG2a allele is expressed in e.g., Balb/c mice.

Alternative namesImmunoglobulin G2a, IgG2a, 1810060O09Rik, Igh-1, Igh-1a
Cell typeB cell
Gene ID380793