Anti-human TNF-α mAb (MT20D9), biotin

Anti-human TNF-α mAb (MT20D9), biotin

3512-6-250

Publications: 111
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Content

Monoclonal antibody MT20D9, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-human TNF-α mAb (MT25C5), unconjugated
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Product specifications

Intended use

This monoclonal antibody enables specific detection of human TNF-α in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

MT20D9 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb MT25C5 (product code 3512-3).

Product details

ProductAnti-human TNF-α mAb (MT20D9), biotin
ApplicationELISpot, FluoroSpot, ELISA
AnalyteTNF-α
AntibodyMT20D9
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant human TNF-α
HostMouse
IsotypeIgG1
ReactivityHuman
SpecificityNative and recombinant human TNF-α
PurificationPurified from in vitro cultures by protein G affinity chromatography.
BiotinylationBiotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MT20D9, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.
Datasheet/ProtocolSDS

TNF-α

Analyte description

Tumor necrosis factor-α (TNF-α) is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID7124
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Highlighted research, Published July 24, 2023

Case report: How researchers used our TNF-α ELISA to evaluate endotoxin contaminations
Bengt Fadeel’s group developed and evaluated a method to uncover endotoxin contaminations using primary human monocyte-derived macrophages and our TNF-α ELISA.