Anti-human TNF-α mAb (MT25C5), unconjugated

Anti-human TNF-α mAb (MT25C5), unconjugated


Publications: 111
Validated for our assays.


Monoclonal antibody MT25C5, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0.00

1 / 1
Anti-human TNF-α mAb (MT20D9), biotin
  • Reactivity:
  • Application:
  • Plates:
  • Code: 3512-6-250
  • Publications:

Product specifications

Intended use

This monoclonal antibody enables specific detection of human TNF-α in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples


MT25C5 is recommended as capture mAb in ELISpot, FluoroSpot, and ELISA in combination with detection mAb MT20D9 (product code 3512-6).

Product details

ProductAnti-human TNF-α mAb (MT25C5), unconjugated
ApplicationELISpot, FluoroSpot, ELISA
ImmunogenRecombinant human TNF-α
SpecificityNative and recombinant human TNF-α
PurificationPurified from in vitro cultures by protein G affinity chromatography.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MT25C5, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.


Analyte description

Tumor necrosis factor-α (TNF-α) is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID7124
Loading publications...

Highlighted research, Published July 24, 2023

Case report: How researchers used our TNF-α ELISA to evaluate endotoxin contaminations
Bengt Fadeel’s group developed and evaluated a method to uncover endotoxin contaminations using primary human monocyte-derived macrophages and our TNF-α ELISA.