Anti-human Granzyme B mAb (MT28), unconjugated

Anti-human Granzyme B mAb (MT28), unconjugated

3486-3-250

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Content

Monoclonal antibody MT28, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Product specifications

Intended use

This monoclonal antibody enables specific detection of human Granzyme B in ELISpot.

Serum/Plasma samples

Recommendation

MT28 is recommended as capture mAb in ELISpot in combination with detection mAb MT8610 (product code 3486-6).

Product details

ProductAnti-human Granzyme B mAb (MT28), unconjugated
ApplicationELISpot
AnalyteGranzyme B
AntibodyMT28
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenRecombinant human Granzyme B
HostMouse
IsotypeIgG1
ReactivityHuman
Specificity

Native and recombinant human Granzyme B

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT28, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

Selecting the appropriate mAbs is key to effectively detecting human Granzyme B, a critical serine protease secreted by cytotoxic T cells and natural killer cells. This mAb guide describes which combination of our Granzyme B mAbs should be used in different immunoassays.

 

Application guide: ELISA

Our human granzyme B ELISA kits can be used to detect the important cytokine in solution, whether it be in serum, cell culture media, or plasma samples.

Capture mAb: MT34B6, unconjugated
Detection mAb: MT28, biotin

GranzymeB_mAb_pair_ELISA

Application guide: ELISpot

Our human granzyme B ELISpot kits can be used to detect the important cytokine directly upon secretion by T cells.

Capture mAb: MT28, unconjugated
Detection mAb: MT8610, biotin

Granzyme B mAb pair ELISpot

Application guide: FluoroSpot

Our human granzyme B FluoroSpot kits can be used to detect the important cytokine directly upon secretion by T cells alongside other analytes in the multiplex format.

Capture mAb: MT34B6, unconjugated
Detection mAb: MT28, biotin

granzyme B FluoroSpot mab pair

 

 

Get inspired!

Make sure to check out how researchers have used Mabtech's human Granzyme B ELISA, ELISpot, and FluoroSpot kits in everything from vaccine development to SARS-CoV-2 research. You can find it all in our Publication database.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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Granzyme B

Analyte description

Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.

Alternative namesGranzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell typeT cell, Tc, Th1, NK cell
Gene ID3002
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