PepPool: CyCMV​+​RhCMV (TP‑UL83​+​Rh112), scanning

PBMC from rhesus macaque (300,000 cells/well) were stimulated with the CyCMV (TP-UL83) + RhCMV (Rh112) scanning pool in Monkey IFN-γ ELISpot. Analysis was done with Mabtech IRIS.
PBMC from rhesus macaque (300,000 cells/well) were incubated overnight in the absence or presence of the CyCMV (TP-UL83) + RhCMV (Rh112) scanning pool in Monkey IFN-γ FluoroSpot. Analysis was done with Mabtech IRIS.

PepPool: CyCMV​+​RhCMV (TP‑UL83​+​Rh112), scanning

3692-1

$510

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Apo ELISA buffer

Intended use

This peptide pool is intended for studying immune responses in rhesus and cynomolgus macaques against CyCMV TP-UL83 and rhCMV Rh112 using immunoassays such as ELISpot, FluoroSpot, and flow cytometry. The peptide pool induces cytokine secretion from antigen-specific CD4 and CD8 T cells. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Description
The CyCMV+RhCMV (TP-UL83+Rh112) scanning pool induces secretion of e.g., IFN-γ from monkey T cells. It consists of 133 peptides derived from cynomolgus macaque cytomegalovirus (CyCMV) Tegument phosphoprotein UL83 (Uniprot ID: G8H1B8) and 133 peptides derived from rhesus macaque cytomegalovirus (rhCMV) Rh112 (Uniprot ID: Q772N2). The peptides are 15-mers overlapping with 11 amino acids and cover the complete proteins. The mean purity of the peptides is 83%.

The peptide pool has been validated in IFN-γ ELISpot and FluoroSpot using PBMC from cynomolgus and rhesus macaques.

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Product details

ProductPepPool: CyCMV+RhCMV (TP-UL83+Rh112), scanning
ApplicationT cell stimulation, ELISpot, FluoroSpot, Flow cytometry
ReactivityNon-human primates
Supplied in

Lyophilized

Contents

Peptide pool with 133 peptides, 25 ug of each peptide. Supplied in two vials (lyophilized).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at -20 °C or below upon receipt. 

PBMC from rhesus macaque (300,000 cells/well) were stimulated with the CyCMV (TP-UL83) + RhCMV (Rh112) scanning pool in Monkey IFN-γ ELISpot. Analysis was done with Mabtech IRIS.
PBMC from rhesus macaque (300,000 cells/well) were stimulated with the CyCMV (TP-UL83) + RhCMV (Rh112) scanning pool in Monkey IFN-γ ELISpot. Analysis was done with Mabtech IRIS.
PBMC from rhesus macaque (300,000 cells/well) were stimulated with the CyCMV (TP-UL83) + RhCMV (Rh112) scanning pool in Monkey IFN-γ ELISpot. Analysis was done with Mabtech IRIS.
PBMC from rhesus macaque (300,000 cells/well) were incubated overnight in the absence or presence of the CyCMV (TP-UL83) + RhCMV (Rh112) scanning pool in Monkey IFN-γ FluoroSpot. Analysis was done with Mabtech IRIS.

Datasheet/ProtocolSDS

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Published: October 28, 2024 | 18 minute read

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CyCMV + RhCMV peptide pool

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