ELISA Flex: Human IgE (ALP)

Standard curve for human IgE ELISA Flex kit.

ELISA Flex: Human IgE (ALP)

3810-1A-6

Publications: 21

Documents

Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	107
Detection mAb	182, biotin
Enzyme conjugate	Streptavidin-ALP enzyme conjugate for ELISpot
Standard	Native human IgE ELISA standard
Buffer/Solution	Standard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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ELISA diluent

Intended use

This ELISA Flex kit is intended for the quantification of human IgE in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Human IgE (ALP)
Application	ELISA
Analyte	IgE
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IgE.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Standard range

0.3-30 ng/ml

Representative standard curve for human IgE ELISA Flex kit using pNPP substrate.

Datasheet/Protocol SDS

Tutorial

11 tips for your first ELISA

Get the most out of your next ELISA experiment and find out how to get reliable results with our checklist.

Tutorial

ELISA kit with HRP or ALP – what to choose?

You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

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IgE

Analyte description	The main physiological function of immunoglobulin E (IgE) is to protect against parasitic infestation. However, IgE is also associated with allergy, and allergen-specific IgE antibodies may trigger allergic reactions in the presence of the allergen. IgE is normally found in low amounts in serum/plasma but is significantly increased in allergic individuals, and the detection of allergen-specific IgE serves as the basis for many in vitro assays for allergy diagnosis.
Alternative names	Immunoglobulin E, IgE
Cell type	B cell
Gene ID	3497

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ELISA Pro: Human IgE