ELISA Pro: Human IgE

ELISA Pro: Human IgE


Publications: 17
Thoroughly validated and include every buffer necessary, for a reliable and reproducible assay.


PlatePre-coated ELISA strip plate (mAb 107)
Detection mAb182, biotin
Enzyme conjugateStreptavidin-HRP
StandardNative human IgE ELISA standard
SubstrateELISA substrate: TMB for HRP
Buffers/SolutionsELISA diluent
Standard reconstitution buffer
Streptavidin-HRP diluent
Wash buffer concentrate
Stop solution
Also providedAdhesive plate covers

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISA Pro kit is intended for the quantification of human IgE in serum, plasma, and cell culture supernatants. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The ELISA diluent included in this kit blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors.


Product details

ProductELISA Pro: Human IgE

This kit is based on a matched pair of mAbs specific for human IgE.

Shipping and Storage


Shipped at ambient temperature.


Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature.

Shelf lifeAt least 12 months from date of receipt.
Standard range0.1-31.6 ng/ml
Sensitivity0.03 ng/ml

The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 5 standard deviations to the mean OD of background wells.

Mean standard curve from repeated runs of human IgE ELISA Pro kit.

Selecting the appropriate mAbs is key to detecting both total and antigen-specific human IgA. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect human IgA antibody-secreting cells.


Application guide: ELISA


Antigen-specific IgE

Detection mAbs:
107/182/101, biotin

IgE Ag

Total IgE

107, unconjugated
Detection mAbs:
182, biotin

IgE total


Application guide: ELISpot


Total IgE

Coating mAb:
107, unconjugated
Detection mAbs:
107/182/101, biotin




Get inspired!

Make sure to check out how researchers have used Mabtech's human IgE ELISA, ELISpot, and FluoroSpot kits in allergy research. You can find it all in our Publication database.

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.
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Analyte description

The main physiological function of immunoglobulin E (IgE) is to protect against parasitic infestation. However, IgE is also associated with allergy, and allergen-specific IgE antibodies may trigger allergic reactions in the presence of the allergen. IgE is normally found in low amounts in serum/plasma but is significantly increased in allergic individuals, and the detection of allergen-specific IgE serves as the basis for many in vitro assays for allergy diagnosis.

Alternative namesImmunoglobulin E, IgE
Cell typeB cell
Gene ID3497