FluoroSpot Path: SARS‑CoV‑2 (Spike+RBD) Human IgG

Product discontinued

This product is no longer available for purchase.
You may be interested in the following alternative:

FluoroSpot Path: SARS-CoV-2 (RBD) Human IgG

Human PBMC from a COVID-19 convalescent individual were incubated with R848+IL-2 (stimulates memory B cells) in tubes for three days. Cells were then extensively washed and added to an FluoroSpot plate (250,000 cells/well) coated with anti-human IgG mAbs. Spike-specific IgG spots were detected with Spike-GAL, i.e., recombinant Spike protein with a GAL peptide tag, followed by anti-GAL-490. RBD-specific spots were detected with RBD-WASP, i.e., recombinant Receptor Binding Domain protein with a WASP peptide t

FluoroSpot Path: SARS‑CoV‑2 (Spike+RBD) Human IgG

Product discontinued

This product is no longer available for purchase.
You may be interested in the following alternative:

FluoroSpot Path: SARS-CoV-2 (RBD) Human IgG

This discontinued product was available in the following format:

1 plate (Product code: FSP-05R-RS1-1)

Components

Plate	Pre-coated FluoroSpot plate (mAbs MT91/145)
Detection mAb	Anti-IgG mAbs (MT78/145), 550
Antigens	Spike-GAL
	RBD-WASP
Fluorophore conjugates	Anti-WASP mAb, 640
	Anti-GAL mAb, 490
Buffers/Solutions	FluoroSpot enhancer
	Reconstitution buffer
Stimuli	R848
	Recombinant human IL-2

Product specifications

Intended use

This FluoroSpot Path kit is intended for the enumeration of B cells secreting human IgG in response to SARS-CoV-2 (Spike and RBD) using the FluoroSpot assay. B cells secreting IgG irrespective of antigen specificity (total IgG) can also be enumerated. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

For memory B cells, we recommend pre-stimulation with R848 and recombinant human IL-2 (both included).

Recommendation

Product details

Product	FluoroSpot Path: SARS-CoV-2 (Spike+RBD) Human IgG
Application	FluoroSpot
Analyte	IgG
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for the Fc part of human IgG.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, fluorophore conjugates, and FluoroSpot enhancer at 4-8 °C upon receipt. Recombinant proteins should be stored at -20 °C or below. Plates may be kept at room temperature.
Shelf life	At least 12 months from date of receipt.

Performance

Please be advised that this product has been discontinued.

Human PBMC from a COVID-19 convalescent individual were incubated with R848+IL-2 (stimulates memory B cells) in tubes for three days. Cells were then extensively washed and added to an FluoroSpot plate (250,000 cells/well) coated with anti-human IgG mAbs. Spike-specific IgG spots were detected with Spike-GAL, i.e., recombinant Spike protein with a GAL peptide tag, followed by anti-GAL-490. RBD-specific spots were detected with RBD-WASP, i.e., recombinant Receptor Binding Domain protein with a WASP peptide tag, followed by anti-WASP-640. Spots were analyzed with Mabtech IRIS.

Product highlights