ELISpot Flex: Human IL‑29 (ALP)

PBMC (500,000 cells/well) were incubated overnight in the absence or presence of 2’3’-cGAMP and the number of cells secreting IL-29 (IFN-λ1) was analyzed by ELISpot. Spots were analyzed using Mabtech IRIS.

ELISpot Flex: Human IL‑29 (ALP)

3570-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT2H7
Detection mAbMT6G4, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot

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ELISpot substrate: BCIP/NBT-plus for ALP

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-29 (IFN-λ1) using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

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Product details

ProductELISpot Flex: Human IL-29 (ALP)
ApplicationELISpot
AnalyteIL-29 (IFN-λ1)
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IL-29 (IFN-λ1).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.
PBMC (500,000 cells/well) were incubated overnight in the absence or presence of 2’3’-cGAMP and the number of cells secreting IL-29 (IFN-λ1) was analyzed by ELISpot. Spots were analyzed using Mabtech IRIS.

PBMC (500,000 cells/well) were incubated overnight in the absence or presence of 2’3’-cGAMP and the number of cells secreting IL-29 (IFN-λ1) was analyzed by ELISpot. Spots were analyzed using Mabtech IRIS.

Datasheet/ProtocolSDS

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IL-29 (IFN-λ1)

Analyte description

Interleukin 29 (IL-29, IFN-λ1) is at type III interferon. Presently, three type III interferons are described: IFN-λ1, IFN-λ2, and IFN-λ3. Similar to type I interferons, IL-29 is mainly produced by monocytes and dendritic cells in response to viral infections. Among other anti-viral effects, IL-29 induces upregulation of MHC class I on the surface of the virus-infected cell.

Alternative namesInterleukin 29, IL-29, IL29, IFN-λ1, Interferon-λ1, IFN-lamba1, IFNL1
Cell typeT cell, Th1, Th17, NK cell
Gene ID282618