ELISA Flex: Monkey Granzyme B (HRP)

Standard curve for monkey granzyme B ELISA Flex kit.

ELISA Flex: Monkey Granzyme B (HRP)

3486M-1H-6

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Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MTAG8
Detection mAb	MT8213, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate
Standard	Recombinant monkey granzyme B ELISA standard
Buffer/Solution	Standard reconstitution buffer

In stock

Delivery 4-9 business days

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Complementary products

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ELISA diluent

Intended use

This ELISA Flex kit is intended for the quantification of monkey granzyme B in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in serum/plasma from other species. The ELISA diluent has been validated using from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Monkey Granzyme B (HRP)
Application	ELISA
Analyte	Granzyme B
Reactivity	Non-human primates
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of granzyme B from non-human primates (NHP).

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Standard range

400-40000 pg/ml

Representative standard curve for monkey granzyme B ELISA Flex kit using TMB substrate.

Datasheet/Protocol SDS

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Granzyme B

Analyte description	Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.
Alternative names	Granzyme B, GzmB, GrB, GrzB
Cell type	T cell, Tc, Th1, NK cell
Gene ID	716518