ELISA Flex: Human IFN‑γ (HRP)

Standard curve for human IFN-γ ELISA Flex kit.
Model of human IFN-γ protein showing the epitopes for monoclonal antibodies 1-D1K (blue) and 7-B6-1 (orange).

ELISA Flex: Human IFN‑γ (HRP)

3420-1H-6

Publications: 259
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Bulk or custom format
An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb1-D1K
Detection mAb7-B6-1, biotin
Enzyme conjugateStreptavidin-HRP enzyme conjugate
StandardRecombinant human IFN-γ ELISA standard
Buffer/SolutionStandard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

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Intended use

This ELISA Flex kit is intended for the quantification of human IFN-γ in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Human IFN-γ (HRP)
ApplicationELISA
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.

Standard range4-400 pg/ml
Standard curve for human IFN-γ ELISA Flex kit.
Representative standard curve for human IFN-γ ELISA Flex kit using pNPP substrate.
Standard curve for human IFN-γ ELISA Flex kit.
Model of human IFN-γ protein showing the epitopes for monoclonal antibodies 1-D1K (blue) and 7-B6-1 (orange).

Tutorial

11 tips for your first ELISA

Get the most out of your next ELISA experiment and find out how to get reliable results with our checklist.

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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458

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