ELISA Flex: Human Granzyme B (HRP)

ELISA Flex: Human Granzyme B (HRP)

3486-1H-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT34B6
Detection mAbMT28, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human granzyme B ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human granzyme B in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

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Product details

ProductELISA Flex: Human Granzyme B (HRP)
ApplicationELISA
AnalyteGranzyme B
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human granzyme B.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range10-1000 pg/ml
Representative standard curve for human Granzyme B ELISA Flex kit using TMB substrate.

Selecting the appropriate mAbs is key to effectively detecting human Granzyme B, a critical serine protease secreted by cytotoxic T cells and natural killer cells. This mAb guide describes which combination of our Granzyme B mAbs should be used in different immunoassays.

 

Application guide: ELISA

Our human granzyme B ELISA kits can be used to detect the important cytokine in solution, whether it be in serum, cell culture media, or plasma samples.

Capture mAb: MT34B6, unconjugated
Detection mAb: MT28, biotin

GranzymeB_mAb_pair_ELISA

Application guide: ELISpot

Our human granzyme B ELISpot kits can be used to detect the important cytokine directly upon secretion by T cells.

Capture mAb: MT28, unconjugated
Detection mAb: MT8610, biotin

Granzyme B mAb pair ELISpot

Application guide: FluoroSpot

Our human granzyme B FluoroSpot kits can be used to detect the important cytokine directly upon secretion by T cells alongside other analytes in the multiplex format.

Capture mAb: MT34B6, unconjugated
Detection mAb: MT28, biotin

granzyme B FluoroSpot mab pair

 

 

Get inspired!

Make sure to check out how researchers have used Mabtech's human Granzyme B ELISA, ELISpot, and FluoroSpot kits in everything from vaccine development to SARS-CoV-2 research. You can find it all in our Publication database.

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 
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Granzyme B

Analyte description

Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.

Alternative namesGranzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell typeT cell, Tc, Th1, NK cell
Gene ID3002