ELISA Flex: Human EBI3 (HRP)

Standard curve for human EBI3 ELISA Flex kit.

ELISA Flex: Human EBI3 (HRP)

3459-1H-6

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Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MT23A14
Detection mAb	MT140, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate
Standard	Recombinant human IL-27 ELISA standard
Buffer/Solution	Standard reconstitution buffer

In stock

Delivery 4-9 business days

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ELISA diluent

Intended use

This ELISA Flex kit is intended for the quantification of human EBI3 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Human EBI3 (HRP)
Application	ELISA
Analyte	EBI3
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human EBI3 subunit of IL-27 and IL-35. The assay will detect both IL-27 and IL-35.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Standard range

15-3000 pg/ml

Representative standard curve for human EBI3 ELISA Flex kit using TMB substrate.

Datasheet/Protocol SDS

Tutorial

2023-09-20

ELISA kit with HRP or ALP – what to choose?

You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial

2022-11-28

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

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EBI3

Analyte description	EBI3 is a protein subunit that forms heterodimers with other proteins in the IL-12 family. IL-27 is a heterodimer composed of the subunits EBI3 and p28. IL-35 is a heterodimer composed of the subunits EBI3 and p35. IL-39 is a heterodimer consisting of the subunits EBI3 and p19. Native IL-39 has not yet been detected in humans. The EBI3 has ca. 30% sequence similarity to IL-12 p40.
Alternative names	Epstein–Barr virus-induced gene3, EBI3, Interleukin-27 subunit beta, IL-27B, IL27B, IL35B
Cell type	Monocyte/MΦ, mDC
Gene ID	10148