ELISA Flex: Equine IL‑10 (HRP)

Standard curve for equine IL-10 ELISA Flex kit.

ELISA Flex: Equine IL‑10 (HRP)

3126-1H-6

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Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MT3515
Detection mAb	MT32AD, biotin
Enzyme conjugate	Streptavidin-HRP enzyme conjugate
Standard	Recombinant human IL-10 ELISA standard
Buffer/Solution	Standard reconstitution buffer

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Delivery 4-9 business days

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ELISA substrate: TMB for HRP

Intended use

This ELISA Flex kit is intended for the quantification of equine IL-10 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in serum/plasma from other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Equine IL-10 (HRP)
Application	ELISA
Analyte	IL-10
Reactivity	Horse
Specificity	This kit is based on a matched pair of mAbs enabling specific detection of equine (horse) IL-10.
Cross-reactivity	The mAbs react with monkey IL-10 and cross-react with IL-10 from human and horse. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Standard range

2-250 pg/ml

Representative standard curve for equine IL-10 ELISA Flex kit using TMB substrate.

Datasheet/Protocol SDS

Tutorial

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

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IL-10

Analyte description	Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.
Alternative names	Interleukin-10, IL-10, IL10
Cell type	T cell, Treg, Monocyte/MΦ, mDC