Anti-mouse IL-5 mAb (TRFK4), biotin

Anti-mouse IL-5 mAb (TRFK4), biotin

3391-6-250

Publications: 39
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Validated for our assays.

Content

Monoclonal antibody TRFK4, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

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Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse IL-5 in immunoassays such as ELISpot, FluoroSpot, and ELISA.

Serum/Plasma samples

Recommendation

TRFK4 is recommended as detection mAb in ELISpot, FluoroSpot, and ELISA in combination with capture mAb TRFK5 (product code 3391-3).

Product details

ProductAnti-mouse IL-5 mAb (TRFK4), biotin
ApplicationELISpot, FluoroSpot, ELISA
AnalyteIL-5
AntibodyTRFK4
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant mouse IL-5
HostRat
IsotypeIgG2a
ReactivityMouse
SpecificityNative and recombinant mouse IL-5
PurificationPurified from in vitro cultures by protein G affinity chromatography.
BiotinylationBiotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration1 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody TRFK4, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.

Find out which analyte combinations we have evaluated in T cell FluoroSpot and which combinations are affected by capture effects or not. 

What is a capture effect?

When capture antibodies with different specificities are coated together, capture of one cytokine may affect the secretion of other cytokines. This is usually more pronounced when studying T cell responses with polyclonal stimuli compared to antigen-specific responses. Capture effects are seldom a problem and can often be counteracted by addition of an anti-CD28 antibody.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IL-5

Analyte description

Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells.  IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells.

Alternative namesInterleukin-5, IL-5, IL5, BCGF-2, Il-5
Cell typeT cell, Th2, NK cell
Gene ID16191
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