Anti-mouse apoA1 mAb (mHDL93), unconjugated

Anti-mouse apoA1 mAb (mHDL93), unconjugated

3750-3-250

Publications: 6
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Validated for our assays.

Content

Monoclonal antibody mHDL93, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-mouse apoA1 mAb (mHDL36), biotin
  • Reactivity:
  • Application:
  • Plates:
  • Code: 3750-6-250
  • Publications:

Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse apoA1 in immunoassays such as ELISA and Western blot.

Serum/Plasma samples

Recommendation

mHDL93 is recommended as capture mAb in ELISA in combination with biotinylated detection mAb mHDL36 (product code 3750-6). The antibody is also suitable for Western blot.

Product details

ProductAnti-mouse apoA1 mAb (mHDL93), unconjugated
ApplicationELISA, Western blot
AnalyteapoA1
AntibodymHDL93
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenNative mouse apoA1
HostRat
IsotypeIgG2a
ReactivityMouse
Specificity

Mouse apoA1

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody mHDL93, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Detection of mouse apoA1 with mAb mHDL93 in Western blot. Mouse serum (0.1 µl) in SDS sample buffer were run on SDS-page / Western blot. ApoA1 was detected using 1 µg/ml mHDL93 (biotinylated) followed by SA-ALP and BCIP/NBT-plus substrate.
Datasheet/ProtocolSDS

apoA1

Analyte descriptionApolipoprotein A1 (apoA1, also known as apolipoprotein-A1, apo A1 and apoA-I) is the main protein component of high density lipoprotein particles (HDL). ApoA1 (28 kD) is mainly produced in the liver and exported into the circulation where it acquires lipids and becomes HDL. High level of apoA1 is regarded as protective against atherosclerosis and cardiovascular disease. Normal concentration of apoA1 in human plasma is approximately 0.8-2.2 g/L.
Alternative names, Alp-1, Apoa-1, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2, Sep2, apo-AI, apoA-I
Gene ID11806
Loading publications...

Tutorial, Published November 14, 2022

Apolipoprotein dilution
Recommendations for Mabtech's apolipoprotein ELISAs

Highlighted research, Published September 27, 2018

Immunological effects of hyperlipidemia
First citations for Mabtech ELISA mouse apoE and apoA1 investigating immunological effects of hyperlipidemia.