Anti-human IgG1 mAb (MTG1218), biotin

3851-14-250

Publications: 3

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Intended use

This monoclonal antibody enables specific detection of human IgG1 in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MTG1218 is recommended as detection mAb in ELISpot in combination with capture mAb MT145 (product code 3850-1) or with coated antigen. The antibody is also suitable for antigen-specific ELISA.

Product details

Product	Anti-human IgG1 mAb (MTG1218), biotin
Application	ELISpot, ELISA
Analyte	IgG1
Antibody	MTG1218
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Native human IgG
Host	Mouse
Isotype	IgG1
Reactivity	Human, Non-human primates
Specificity	Human IgG1
Cross-reactivity	No cross-reactivity (less than 0.1%) with human IgG2, IgG3, IgG4, IgA1, IgA2, IgE, IgM, IgD, or with IgG from mouse and cow.
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MTG1218, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Datasheet/Protocol SDS

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IgG1

Analyte description	Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in serum, making up approximately 80% of all serum Immunoglobulins. In humans, there are four subclasses of IgG with the highest serum concentrations of IgG1, followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. IgG1 binds to Fc receptors with high affinity and may also activate the complement system.
Alternative names	Immunoglobulin G1, IgG1
Cell type	B cell
Gene ID	3500

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ELISpot Flex: Human IgG1 (ALP)