Anti-human IgE mAb (107), unconjugated

3810-3-250

Publications: 21

Documents

Questions?

Validated for our assays.

Content

Monoclonal antibody 107, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

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Delivery 4-9 business days

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Anti-human IgE mAb (182), biotin

Product specifications

Intended use

This monoclonal antibody enables specific detection of human IgE in immunoassays such as ELISA and ELISpot.

Serum/Plasma samples

Recommendation

107 is recommended as capture mAb in ELISA in combination with detection mAb 182 (product code 3810-6) and with detection mAbs 107/182/101 (product code 3810-8) for ELISpot.

Product details

Product	Anti-human IgE mAb (107), unconjugated
Application	ELISA, ELISpot
Analyte	IgE
Antibody	107
Conjugate	Unconjugated
Clonality	Monoclonal
Immunogen	Native human IgE
Host	Mouse
Isotype	IgG1
Reactivity	Human
Specificity	Human IgE
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Concentration	1 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody 107, unconjugated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C.
Shelf life	At least 18 months from date of receipt.

Documents

Datasheet/Protocol
SDS

Human IgE mAb guide

Selecting the appropriate mAbs is key to detecting both total and antigen-specific human IgA. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect human IgA antibody-secreting cells.

Application guide: ELISA

Antigen-specific IgE

Coating:
Antigen
Detection mAbs:
107/182/101, biotin

Total IgE

Coating:
107, unconjugated
Detection mAbs:
182, biotin

Application guide: ELISpot

Total IgE

Coating mAb:
107, unconjugated
Detection mAbs:
107/182/101, biotin

Get inspired!

Make sure to check out how researchers have used Mabtech's human IgE ELISA, ELISpot, and FluoroSpot kits in allergy research. You can find it all in our Publication database.

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Publications (21)

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Analyte information

IgE

Analyte description	The main physiological function of immunoglobulin E (IgE) is to protect against parasitic infestation. However, IgE is also associated with allergy, and allergen-specific IgE antibodies may trigger allergic reactions in the presence of the allergen. IgE is normally found in low amounts in serum/plasma but is significantly increased in allergic individuals, and the detection of allergen-specific IgE serves as the basis for many in vitro assays for allergy diagnosis.
Alternative names	Immunoglobulin E, IgE
Cell type	B cell
Gene ID	3497