Anti-bovine IgG mAb (MT134), unconjugated

Anti-bovine IgG mAb (MT134), unconjugated

3150-3-250

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Content

Monoclonal antibody MT134, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-bovine IgG mAb (MT391), biotin


Product specifications

Intended use

This monoclonal antibody enables specific detection of bovine IgG in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT134 is recommended as capture mAb in ELISpot and ELISA in combination with detection mAb MT391 (product code 3150-6).

Product details

ProductAnti-bovine IgG mAb (MT134), unconjugated
ApplicationELISpot, ELISA
AnalyteIgG
AntibodyMT134
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenNative bovine IgG
HostRat
IsotypeIgG1
ReactivityCow
Specificity

Bovine (cow) IgG

Cross-reactivity

Cross-reacts with IgG from human (86% cross-reactivity), horse (6% cross-reactivity) and sheep (1% cross-reactivity). For more information, please check the Veterinary cross-reactivity guide.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibody MT134, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgG

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG
Cell typeB cell
Gene ID281850, 281851, 281852
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ELISA Flex: Bovine IgG (HRP)